Cryopreservation of Tobacco BY-2 Cells.
نویسندگان
چکیده
منابع مشابه
Isolation of cortical MTs from tobacco BY-2 cells.
We isolated the cortical microtubules (CMTs) from tobacco BY-2 cells to identify their components. By centrifugation of protoplasts homogenized in the presence of taxol, a MT-stabilizing reagent, in a density gradient of Percoll, we obtained membranous vesicles to which MTs forming a sheet-like bundle were attached. Rhodamine-conjugated Ricinus communis agglutinin I (RCA-I), a lectin that bound...
متن کاملOptimization of human peripheral blood mononuclear cells (PBMCs) cryopreservation
Cryopreservation is the method of choice for long term storage of human PBMCs. This study was designed to compare the different combinations of variables affecting the cryopreservation of PBMCs samples. The viability of PBMCs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with ...
متن کاملThe role of phenolic compounds in growth improvement of cultured tobacco cells after exposure to 2-D clinorotation
Previous studies have confirmed that the growth and development of plants are entirely dependent on the gravitational acceleration of the Earth. So far, most of the studies on the plant response to the Earth gravity have focused on the geotropism of root tip of higher plants<span lang="AR-SA" dir="RTL...
متن کاملInm-2: New Approaches in Cryopreservation
Cryopreservation of embryos and gametes has become an indispensible service for infertility treatment. Vitrification is now a prevalent technique for cryopreservation in assisted reproductive technology (ART) replacing, in many cases, conventional slow cooling methodology. Fertility preservation in female patients has become a very topical issue. Various malignant and nonmalignant diseases have...
متن کاملCryopreservation of Cultured Plant Cells
Undifferentiated cell cultures of higher plants are important genetic resources used extensively in cytological, physiological, and molecular biological studies. The development of a reliable and cost-effective method for the maintenance of cultured cells is required to minimize the risk of losing cell lines to disease, contamination, and technical errors, as well as the risk of changes in morp...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant tissue culture letters
سال: 1996
ISSN: 1884-7706,0289-5773
DOI: 10.5511/plantbiotechnology1984.13.331